عنوان
Simultaneous determination of β-sitosterol and cholesterol in food samples by using solid phase microextraction fibers based on monolithic moleculary imprinted polymer followed by HPLC–UV
نویسنده (گان)
Kardani,Fatemeh; mirzajani,Roya
چکیده مقاله
In this paper, an efficient and sensitive analytical method monolithic solid phase microextraction (SPME) fiber on the basis of moleculary imprinted polymer coupled to reverse phase high performance liquid chromatography (RP-HPLC) - UV detection analysis was developed for extraction and determination of β-sitosterol and cholesterol in food samples. To obtain optimum extraction performance, several extraction parameters including extraction and desorption solvent, starring rate, extraction and desorption time were investigated and discussed. Under the optimized extraction conditions, the limits of detection (S/N = 3) of the proposed method were 0.03 and 0.05µg L−1, for of β-sitosterol and cholesterol respectively. Satisfactory linearity was achieved in the range of 0.1–20µg L−1for analytes with the correlation coefficients (r) above 0.9975. A method precision was performed by Intra-day and inter-day precisions taste which was achieved for the analysis of β-sitosterol and cholesterol in the range of 1.1–1.9%. This procedure was successfully applied with satisfactory results to the determination β-sitosterol and cholesterol in spiked milk, egg yolk and olive oil samples. The relative mean recoveries of oil samples ranged from 92.0% to 101.0%.

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