عنوان
Interaction of CT-DNA/BSA With Ethylendiamine 2-pyridinecarboxcylatopaladium(II) ninarte and anti- tumor activity
نویسنده (گان)
Jamgohari, nasimeh;Mansouri-torshizi,Hassan
چکیده مقاله
Cis-Diamminedicholoroplatinum (II) (cisplatin), first identified as antitumor drug in late 1960. Thousand’s analog of this compound has been synthesized, characterized and tested for their antitumor activities. It has been reported that many active complexes could react with DNA and inhabit its synthesis1. Much success has been achieved with platinum-based chemotherapeutic agents, i.e. through interactions with DNA. The long-term application of Pt complexes is thwarted by issues, leading scientists to examine other metals such as palladium which could exhibit complementary modes of action (given emphasis wherever known). Over the last 10 years several research groups have focused on the application of an eclectic array of palladium complexes (of the type PdX2L2, palladacycles and related structures) as potential anti-cancer agents2. A new palladium(II) complex of formula [Pd(en)(2-py)]NO3 (where en is 1,2- diaminoethane and 2-py is 2-pyridinecarboxylate anion) have been synthesized and characterized by elemental analysis, conductivity measurements, and UV-Vis, FT-IR and 1H NMR spectroscopic techniques. This complex has been interacted with bovine serum albumin (BSA) and calf thymus DNA using UV-Vis spectroscopy in 30 mmol/L Tris-HCl buffer of pH = 7.00 at tow temperatures of 300 and 310K. In these studies, Kapp, apparent association constant is 1.3 ×104 M–1 and 1.9 ×105 M–1 for DNA and 1.5 ×104 M–1 and 1.1 ×105 M–1 for BSA at 300 and 310 K respectively. These studies indicate that the bove compound can interact with DNA as well as BSA and the concentration of that in the midpoint of transition from native to denatured DNA or BSA, [L]1/2, is increased by increasing from 0.034 mM to 0.038 mM for DNA and from 0.023 mM to 0.036 mM for BSA at 300 and 310 K respectively. The in vitro antitumor properties of the Pd(II) complex were carried out with human tumor cell line K562. The 50% cytotoxic concentration (Cc50) of the complex was determined 32µM. Moreover, using Kapp value, the thermodynamic parameters (ΔG°, ΔS° and ΔH°) have also been calculated.

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